The Evans Cycles store in Crawley town centre will close on Saturday (September 12).
A spokesman for the chain said no staff at The Broadway site would lose their jobs.
He said the lease on the building was up for renewal and its Gatwick Warehouse site was nearby in Manor Royal.
The large unit in The Broadway opened some ten years ago.
The spokesman said: “The Crawley shop was one that we took over from another owner at around the same time that we moved into our Gatwick premises – so at that point we only had one shop in the area.
“It has taken many years for the Gatwick showroom to establish its presence, and with the lease coming to an end on our Crawley shop it makes sense for both us and our customers to focus on the Gatwick shop.”
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