Roads in Horsham, Crawley and Mid Sussex have been revealed as deer collision hotspots by the Deer Initiative.
Forest Road, which runs to Pease Pottage, and roundabouts on the A24 have been identified in the Horsham area.
In Crawley, the Deer Initiative has warned motorists to take extra care on the M23 and local roads.
And drivers should look out for deer on the A23 and A272 in Handcross, Cuckfield and Haywards Heath, the partnership said.
There are up to 75,000 deer vehicle collisions each year in the UK, resulting in 400 to 700 human injuries and several human fatalities each year.
The Deer Initiative estimated that over 14,000 vehicles in the UK will incur significant damage from these incidents.